The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology and microbiology. Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to
Feb 14, 2008 · Gene expression studies have been greatly facilitated by the employment of proteins like β-glucuronidase (GUS), green fluorescent protein (GFP), and firefly luciferase (LUC) as
発表年:Plant Biology · 2003著者: N C A De Ruijter · John Verhees · W Van Leeuwen · A R Van Der Krol提携:Wageningen University and Research Centre詳細情報: Glucuronidase · Luciferase · Light emission · Beta-glucuronidase activity · Reporter gene
Jun 07, 2005 · The GFP-GUS fusion vectors are based on pCambia 1304, which has an engineered, fused GFP-GUS orf (Figure (Figure1B). 1B). The GFP was changed to redshifted GFP to enhance detection [ 24 ]. A PCR product was made using the oligonucleotide primers rsGFP-F (5’CTGTTCCATGGCCAACACTTGTCACTACTTTC A CTTATGGTGTT) and rsGFP-reverse
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Jun 25, 2007 · Gus is an enzyme ( beta-glucuronidase), so you can check for the activity of this enzyme. GFP is a protein with fluorescence capacities (it’s from a jellyfish, Aequorea victoria).
Sep 01, 1999 · GFP and GUS production were monitored during several stages of the life cycle of P. palmivora to evaluate their use in molecular and physiological studies. 40% of the GFP transformants produced the GFP to a level detectable by a confocal laser scanning microscope, whereas 75% of the GUS transformants produced GUS.
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GUS and GFP transformation of the biocontrol strain Clonostachys rosea IK726 and the use of these marker genes in ecological studies. Marker genes were introduced in the biocontrol strain Clonostachys rosea IK726 (IBT 9371) as a tool for monitoring the strain in ecological studies.
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Evaluation and Comparison of the GUS, LUC and GFP Reporter System for Gene Expression Studies in Plants N. C. A. de Ruijter Lab. of Plant Cell Biology, Wageningen University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands
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1997a). The DR5 promoter driving reporter genes like b-glucuronidase (GUS), green ﬂuorescent pro- tein (GFP) or red ﬂuorescent protein (RFP) has been widely utilized in Arabidopsis, maize, soybean, and tomato to study auxin distribution and response at a
発表年:Plant Cell Reports · 2013著者: Yiru Chen · Yordan S Yordanov · Cathleen Ma · Steven H Strauss · Victor Busov提携:Michigan Technological University · Oregon State University詳細情報: Phloem · Auxin
ニダーゼ（GUS）遺伝子、green fluorescent protein（GFP）遺伝子の3種 がある。その中でルシフェラーゼは、高感度で定量性にも優れていることが 知られている。また、ルシフェラーゼの基質であるルシフェリンは植物体に
The GUS gene has been commonly used in plants but luciferase and GFP are becoming more common. A common reporter in bacteria is the E. coli lacZ gene, which encodes the protein beta-galactosidase. This enzyme causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal.
Evaluation and Comparison of the GUS, LUC and GFP Reporter System for Gene Expression Studies in Plants Article in Plant Biology 5(2) · March 2003 with 1,882 Reads How we measure ‘reads’
Gus Gene Assay In Transformed Tissues In order to identify transformed cells or plants that have been growing on a selective medium, it is necessary to have an easily assayable reporter gene. The most useful reporter genes encode an enzyme activity not found in the organism being studied.
74″ ubiquitous expression of a nuclear-localized GFP and β-glucuronidase (GUS) fusion 75″ protein (pHTR5:NLS-GFP-GUS; (Ingouff et al., 2017), henceforth, NLS-GFP). Primary 76″ transgenic plants (T1 seedlings) were selected via resistance to the herbicide 77″ glufosinate and investigated for loss of GFP signal in the root tips of five-day-old
Author: Ward Decaestecker, Rafael Andrade Buono, Marie Pfeiffer, Nick Vangheluwe, Joris Jourquin, Mansour Ka
レポーター遺伝子（レポーターいでんし）とは、ある遺伝子が発現しているかどうかを容易に判別するために、その遺伝子に組換える別の遺伝子のこと。 緑色蛍光タンパク質（gfp）が有名。. 組換えdna技術によって作成された組換え遺伝子が、いつどこでどのくらいできているのかを比較的簡単
Plasmid pGFPGUSPlus from Dr. Claudia Vickers’s lab contains the inserts GFP and GUSPlus and is published in Biotechnol Lett. 2007 Nov;29(11):1793-6. Epub 2007 Aug 9. This plasmid is available through Addgene.
マーカー遺伝子（マーカーいでんし、marker gene）とは、遺伝子組み換え生物を作成する際、組み換えに成功した生物をスクリーニングするための目印として導入する遺伝子である。 選択マーカー (en:Selectable_marker) ともいう。. 多くの場合、遺伝子導入が成功した形質転換体と非形質転換体では
GUS activity staining — a powerful tool in plant molecular biology. Abstract. β-Glucuronidase (GUS) is a very versatile reporter of gene expression that is frequently used in plant molecular biology.
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Mapping the IAA Distribution in the Arabidopsis Root Apex. Since the QC cells contain very high IAA levels, it is difficult to determine whether the very high IAA concentrations in Q0990 reflect further increases in IAA concentration toward the apex, compared with pWOL:GFP and J2501, or are caused by the QC
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Apr 12, 2010 · I am working with a plant and I want to use of Gus and GFP together to determine the promoter and protein expression and localization. I would mention that I didnt use of my gene promoter, I used of another promoter to overexpress my gene. Now the question is how can I fuse 2 reporter gene to get the best result. which one at first?
遺伝子導入においていろいろな分野でgfpが利用されているのは何故ですか？ 遺伝子導入においていろいろな分野でgfpが利用されているのは何故ですか？どなたか教えてください。おねがいします。 「遺伝子導入が上手
Green Fluorescent Protein (GFP) Martin Chalfie, Osamu Shimomura and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 December 2008 for their discovery and development of the green fluorescent protein. The GFP is composed of 238 amino acids, originally isolated from the jellyfish Aequorea victoria that
Jul 08, 2015 · GFP tagging (Green Fluorescent Protein fusion) – This lecture explains about the green fluorescent protein tagging technique and the use of GFP
Aug 15, 2013 · The global GFP–GUS expression driven by VIN in transgenic tomato plants followed the same biphasic global expression pattern previously described for the endogenous VIN gene (Estornell et al., 2009).Global GUS activity, as determined fluorometrically and by tissue staining, was low in stage II of fruit development, but increased at the start of stage III of fruit expansion (stage IIIa).
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Taxonomy. x; UniProtKB. Protein knowledgebase. UniParc. Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects.
protéine fluorescente verte (green fluorescent protein en anglais ou GFP), luciférase, émettant une lumière jaune; l’activité enzymatique avec: le gène GUS, codant une enzyme (la bêta-glucuronidase) qui colore en bleu les cellules où il est actif, mais il est létal
These GUS 8.4 GFP transgenic mice express red-shifted Green Fluorescent Protein under the direction of the mouse Gnat3 gene promoter. Taste receptor cells from circumvallate papillae and retinal bipolar cells (type 7 ON cone bipolar cells and weakly in rod bipolar cells) fluoresce green.
das gfp-Gen aus Aequorea victoria, codiert für ein Grün fluoreszierendes Protein (GFP) das gusA-Gen aus Escherichia coli, codiert für eine β-Glucuronidase (GUS) (früher bekannt als uidA-Gen) das lacZ-Gen aus Escherichia coli, codiert für eine β-Galactosidase (β-Gal), meistens zur Blau-Weiß-Selektion
Promoter:GFP (or GUS) lines are used to reveal the expression pattern of genes. But can they also be used in short term treatments?
laser scanning microscope, whereas 75% of the GUS transformants produced GUS. GFP could be visualised readily in swimming zoospores and other developmental stages of P. palmivora cells. For high magnification microscopic studies, GFP is better visualised and was superior to GUS. In contrast, for macroscopic examination, GUS was superior.
Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.
Intriguing expression patterns of 35S‐GFP and phas‐GUS transgenes in M. truncatula. The progressive loss of GFP expression in aerial parts as the plant aged was dramatic and occurred in each of the four transgenic plants studied. Consequently, transgene copy number does not appear to be a major factor.
Before staining, seedlings were fixed in 90% cold acetone for 1 h on ice, then washed in phosphate buffer for 1 h at room temperature. Seedlings were stained for 4–5 h (CYCB1;1::GUS) or overnight (pPILS6::GUS-GFP). To quantify the signal intensity, we defined a region of interest (ROI) capturing the most representative signal distribution.
The β-glucuronidase (GUS) reporter gene and a gene encoding the green fluorescent protein (GFP) were, in separate experiments, integrated into the genome of IK726 using the Hygromycin B (HygB) resistance gene as selective marker.
otides or PCR products. A β-glucuronidase (GUS)/green florescent protein (GFP) fusion gene was efficiently silenced in transient assays using a pAmiR169d-derived construct targeting a GFP sequence. 5′ RACE showed that the target GFP transcript was cleaved precisely at the expected position across nucleotides 10 and 11 of the amiRNA. Thus,
Introduction. Green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a 27-kDalton monomer protein consisting of 238 amino acids which is naturally fluorescent (1). Its role is to transduce, by energy transfer, the blue chemiluminescence of another protein, aequorin, into
gfp がよく使われるのは？ gfp の蛍光団はアミノ酸残基のみから成り (fig. 2)、その形成も自発的に起こり、補因子を必要としない（蛋白質が単 独で光る）ため、汎用性が高い（例えばホタルの発光を担うルシフェラーゼは、atp を用いてルシフェリンを酸化する
INTRODUCTION. GUS is useful primarily as a tag to address nuclear localization, whereas GFP is more versatile. Moreover, GFP is detectable directly in living cells, whereas GUS is only detected indirectly by staining of fixed tissue. This may lead to artifacts or it may obscure problems with protein solubility.
発表年:CSH Protocols · 2007著者: Albrecht G Von Arnim
The effect of sirtinol seemed to be somewhat delayed when compared to auxin effects, but prolonged treatments had the same impact on the induction of PIN gene expression as auxins, as shown, for example, by the upregulation of PIN4::GUS and PIN1:GFP .
如何利用gus报告基因检测所需基因 标记基因通常用来检验重组DNA载体转化成功与否，或者检测目的基因在植物细胞或组织中的定位。 常用的标记基因是一些抗生素抗性基因，如卡那霉素抗性基因、潮霉素标
Expression of the GUS Reporter in Tomato Fruits. Nevertheless, transient expression data provided the initial evidence that the Gateway cloning framework was compatible with gene expression in tomato, a prerequisite for in-depth analysis of promoter activity in stable transgenic plants.
発表年:Plant Physiology · 2009著者: Ana Fernandez · Nicolas Viron · Moftah Alhagdow · Mansour Karimi · Matthew O Jones提携:Ghent University詳細情報: Functional analysis · Green fluorescent protein · Plant physiology · Biomarker · Zinc fi
In order to select GUS and GFP transformants that resembled the wildtype strain, growth rate, production of enzymes and of metabolites of four GUS positive and six GFP positive transformants were tested in vitro. In addition, the biocontrol efficacy against disease caused by seed-borne Fusarium culmorum was evaluated on barley grown in sand.
2.1 gfp在分子生物学上的应用 2.1.1 gfp作为报告基因 报告基因是一种编码可被检测的蛋白质或酶的dna，如传统的荧光素酶 （lux）基因和β-葡萄糖苷酶（gus）基因。gfp作为基因报告可用来 检测转基因效率，把gfp基因连接到目的基因的启动子之后，通过测定 gfp的荧光
감사합니다. 합성된 유전자를 미생물에 삽입 후 그 미생물을 식물에 감염시킨다. β-glucuronidase란? 거스 유전자는 주로 식물에 사용되는 표지유전자임. β-glucuronidase를 암호화함. 형광 측정법 2. 기관이나 조직단위로 발현부위를 확인 가능 2014101886 원예생명공학과 정환용 2014101866 원예생명공학과
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is finding wide use as a genetic marker that can be directly visualized in the living cells of many heterologous organisms. We have sought to express GFP in the model plant Arabidopsis thaliana , but have found that proper expression of GFP is curtailed due to aberrant mRNA processing.
To express your gene with alternative promoters, or with other selectable markers for stable-cell generation, consider the pTracer™ vectors series. These vectors express the Cycle 3 Green Fluorescent Protein (Cycle 3 GFP) which has spectral excitation and emission wavelengths identical to wild-type GFP.
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GFP cDNA using oligonucleotides 5 -CTCGAGGGAGGCGGTGGAG-GCATG-3 and 5-GTCGACCTTGTACAGCTCGTCCATGC-3 and pH35WG (G.H. and H.T., unpublished) as a template. The resultant GFP cDNA was cloned into the pGEM T-Easy vector (pGEM GFP). The GFP cDNA fragment was excised by XhoI and SalI and re-inserted into the XhoI site of pGEM GFP.
Jun 26, 2009 · In order to determine whether auxin acts as a positional determinant regulating cell specification during megagametogenesis in Arabidopsis, we followed the distribution of auxin by DR5-driven expression in the synthetic reporters DR5::GFP and DR5::GUS (GFP is green fluorescent protein, and GUS is β-glucoronidase) (14, 15). Auxin signaling
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Agrobacterium-mediated Genetic Transformation of Phalaenopsis bellina Using GFP and GUS Reporter Genes Mahmood Maziah* and Chew Yee Chern Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia *E-mail: [email protected] ABSTRACT
Historia. Osamu Shimomura, en los inicios de la década de 1962, fue el primero en aislar la GFP a partir de la Aequorea victoria e identificar qué parte era responsable de la fluorescencia. Junto a Frank Johnson, de la Universidad de Washington, aisló una proteína fluorescente dependiente del calcio, a la que llamó aequorina, nombre derivado de la medusa con la que trabajaban.
The expression of YUCp:GFP-GUS transgenes in the root apex epidermis in presence of Al and either ACC or/and AVG. Four-day old transgenic YUCp:GFP-GUS seedlings were pre-treated with 1 μM AVG or 1 μM ACC for 1 day when used in co-treatment, then the seedlings were treated with 1 μM AVG or 1 μM ACC in the presence or not of 25 μM AlCl 3 for
The apparent discrepancy between the increased level of GUS or GFP activity seen in the age1 roots treated with 10 −8 M IAA and the lack of response in GUS or GFP transcript levels is likely due to the limited number of cells showing increased BA-driven expression.
発表年:The Plant Cell · 1998著者: Yutaka Oono · Qianhong G Chen · Paul J Overvoorde · Claudia Kohler · Athanasios T
The current challenge, now that two plant genomes have been sequenced, is to assign a function to the increasing number of predicted genes. In Arabidopsis, approximately 55% of genes can be assigned a putative function, however, less than 8% of these have been assigned a function by direct experimental evidence. To identify these functions, many genes will have to undergo comprehensive
発表年:Plant Physiology · 2003著者: Mark D Curtis · Ueli Grossniklaus詳細情報: Agrobacterium · Gene expression · Genome · Gram-negative bacteria · Rhizobiaceae
Trichoderma harzianum was cotransformed with genes encoding green fluorescent protein (GFP), β-glucuronidase (GUS), and hygromycin B ( hygB ) resistance, using polyethylene glycol-mediated transformation. One cotransformant (ThzID1-M3) was mitotically stable for 6 months despite successive subculturing without selection pressure. ThzID1-M3 morphology was similar to that of the wild type